Thermo Fisher Scientific Calreticulin Polyclonal Antibody
Human Calreticulin 단백질을 인식하는 Rabbit Polyclonal Antibody. WB, IHC, ELISA, IP 등 다양한 응용에 적합. 보존제 무첨가로 고순도 단백질 A 및 항원 친화 크로마토그래피 정제. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:10,000–1:20,000 |
| ELISA | 1:5,000–1:10,000 |
| Immunoprecipitation (IP) | 1–4 µL/mg of lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant Human Calreticulin protein (Met1–Ala413) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A, Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2787723 |
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%–0.1%).
This antibody has specificity for Human Calreticulin.
Target Information
Calr (Calreticulin) is a calcium-binding chaperone that promotes folding, oligomeric assembly, and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle.
Calr, a lectin, interacts transiently with almost all monoglucosylated glycoproteins synthesized in the ER. It also interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export.
Additionally, Calr may be involved in maternal gene expression regulation and oocyte maturation via calcium homeostasis regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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