Thermo Fisher Scientific HDAC1 (near C-terminus) Polyclonal Antibody
HDAC1 단백질의 C-말단 근처를 인식하는 Rabbit Polyclonal 항체로, WB, IHC, ELISA, IP에 사용 가능. 인간, 마우스, 랫드, 영장류에 반응. 고순도의 affinity chromatography 정제 제품으로, -20°C에서 보관. 연구용 전용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (IHC) | 1:100–1:500 |
| ELISA | 1:20,000–1:60,000 |
| Immunoprecipitation (IP) | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | HDAC1 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human HDAC1. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.59 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 50% glycerol, 0.15M NaCl |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid freeze/thaw cycles. Centrifuge if not completely clear after standing at room temperature.
Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This antibody is specific towards HDAC1. A BLAST analysis suggests cross-reactivity with Human, Mouse, Rat, and Primate based on 100% sequence homology. Cross-reactivity with HDAC1 from other sources has not been determined.
Target Information
Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression.
HDAC1 belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex.
It interacts with the retinoblastoma tumor-suppressor protein, forming a complex crucial for controlling cell proliferation and differentiation.
Together with metastasis-associated protein-2, HDAC1 deacetylates p53 and modulates its effect on cell growth and apoptosis.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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