Thermo Fisher Scientific Phospho-IKK alpha/beta (Ser180, Ser181) Polyclonal Antibody
IKK alpha/beta의 Ser180/181 인산화 상태를 특이적으로 인식하는 Rabbit Polyclonal 항체. Western blot, IHC, ICC 등 다양한 응용에 적합. Human, Mouse, Rat 시료에 반응. 고순도 정제 및 안정적 저장용 PBS buffer 구성.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:200 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human CHUK (Accession O15111), corresponding to amino acid residues around phosphorylated Ser180. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816169 |
Product Specific Information
This antibody detects endogenous levels of IKK alpha/beta only when phosphorylated at Serine 180/181.
Target Information
IKK alpha/beta is encoded by CHUK (conserved helix-loop-helix ubiquitous kinase) and IKKB (inhibitor of kappa light polypeptide gene enhancer in B-cells) located on chromosomes 10 and 8 in humans.
Upon activation of the NF-κB pathway (e.g., by TNF alpha), IKK alpha and IKK beta, together with the regulator NEMO (IKK gamma), phosphorylate IκB proteins. This leads to their ubiquitin-mediated degradation and the subsequent activation and nuclear translocation of NF-κB.
Phosphorylation at serine 176 and 180 in the activation loop induces conformational changes required for kinase activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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