Thermo Fisher Scientific pro-Caspase 3 Monoclonal Antibody (31A893)
Human 및 Mouse 반응성을 가진 pro-Caspase 3 단클론 항체로, Western blot, IHC, ICC에 사용 가능. Recombinant full-length human Caspase-3를 면역원으로 제작. 단백질 G 정제, 액상 형태, 1.0 mg/mL 농도. 세포 사멸 연구에 적합.
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Applications 및 Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 2 µg/mL | View 3 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5 µg/mL | - |
| Immunocytochemistry (ICC/IF) | 2 µg/mL | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 31A893 |
| Immunogen | Recombinant full-length human Caspase-3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2070045 |
Product Specific Information
Suggested positive control: HL-60 or staurosporine-treated apoptotic HeLa cells.
Target Information
Caspases are a family of cysteine proteases that play key roles in programmed cell death (apoptosis). Each caspase precursor includes a prodomain and catalytic subunits. Activation occurs via cleavage adjacent to aspartate residues, forming an active a2b2 tetramer enzyme.
Caspase-3, also known as Yama, CPP32, or apopain, encodes a 32 kDa protein that cleaves substrates such as poly(ADP-ribose) polymerase (PARP), DNA-PK, actin, GAS2, and procaspase-6. Activation involves cleavage at Asp-28/Ser-29 and Asp-175/Ser-176, generating 17 kDa and 12 kDa subunits.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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