Thermo Fisher Scientific CD284 (TLR4) Monoclonal Antibody (HTA125), Super Bright 436, eBioscience
인간 TLR4 인식용 HTA125 단클론 항체로, Super Bright 436 형광체가 결합된 제품입니다. Flow cytometry에 최적화되어 있으며, 405 nm 레이저로 여기되어 436 nm에서 방출됩니다. 면역반응 및 선천면역 연구에 적합합니다.
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Thermo Fisher Scientific CD284 (TLR4) Monoclonal Antibody (HTA125), Super Bright 436, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Flow Cytometry (Flow) | 5 µL (0.5 µg)/test | View 6 publications |
| Neutralization (Neu) | - | View 4 publications |
Product Specifications
| Item | Description |
|---|---|
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), Super Bright™ 436, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | HTA125 |
| Conjugate | Super Bright™ 436 |
| Excitation / Emission Max | 413 / 431 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Wet ice |
| RRID | AB_2744815 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- eFluor 450
- Functional Grade
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 600
- Super Bright 645
- Request custom conjugation
Product Specific Information
The HTA125 monoclonal antibody reacts with human Toll-like receptor 4 (TLR4). This receptor family is characterized by leucine-rich repeats and a cytoplasmic domain similar to IL-1 receptor. TLR4 associates with MD-2 and CD14 for LPS recognition and signaling, and is expressed by peripheral blood monocytes.
For optimal staining of TLR4, use the lysed whole blood protocol rather than Ficoll-gradient prepared cells, as the latter reduces staining intensity.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Pre-diluted and tested on human TLR4/MD2-transfected cells
- Recommended Usage: 5 µL (0.5 µg) per test in 100 µL final volume
- Excitation: 405 nm
- Emission: 436 nm
- Laser: Violet laser
When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401-75) to minimize non-specific polymer interactions.
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
TLR4 is a key receptor in the Toll-like receptor family, essential for pathogen recognition and activation of innate immunity. It is expressed in placenta and myelomonocytic leukocytes. TLR4 forms a complex with CD14 and LY96 for LPS signal transduction, mediating cytokine production for immune response. Mutations in TLR4 can affect LPS responsiveness. It is expressed by monocytes, a subset of B-cells, and placenta.
For Research Use Only. Not for use in diagnostic procedures.
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