Thermo Fisher Scientific RAD51 Monoclonal Antibody (RAD51, 2702)
인간 RAD51 단백질을 표적으로 하는 마우스 단클론 항체로, DNA 복구 연구에 적합합니다. IHC, Flow Cytometry, Peptide Array 등 다양한 응용 가능. 단백질 A/G로 정제된 액상 형태, 보존제 무첨가. 연구용으로만 사용.
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Applications
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1–2 µg/mL
Flow Cytometry (Flow)
- Tested Dilution: 1–2 µg/mL
Peptide Array (Array)
- Tested Dilution: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | RAD51, 2702 |
| Immunogen | Recombinant fragment of human RAD51 protein (around aa 1–134) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C (preferred) |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For IHC (PFA fixed): Incubate antibody for 30 min at room temperature.
- Formalin-fixed tissues require heating in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 min at 95°C, then cooling at room temperature for 20 min.
Target Information
RAD51 is essential for homologous strand exchange, a crucial step in DNA repair through homologous recombination. It binds both single- and double-stranded DNA and exhibits DNA-dependent ATPase activity. RAD51 facilitates the recognition of homology and strand exchange between DNA partners, forming a joint molecule between a processed DNA break and its repair template. It forms nucleoprotein filaments in an ATP-dependent manner, necessary for homology search and strand exchange. Mutations in RAD51 are associated with breast cancer, mirror movements 2, and Fanconi anemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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