Thermo Fisher Scientific RAD51 Monoclonal Antibody (RAD51, 2702)
RAD51 단백질을 인식하는 Mouse monoclonal antibody로, Human 시료에 반응합니다. IHC, Flow Cytometry, Peptide Array 등에 사용 가능하며, Protein A/G로 정제된 액상 형태입니다. DNA 복구 관련 연구용으로 적합합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/mL |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | RAD51, 2702 |
| Immunogen | Recombinant fragment of human RAD51 protein (around aa 1–134) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage conditions | 4°C |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed):
Incubate antibody for 30 min at room temperature.
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at room temperature for 20 minutes.
Target Information
RAD51 plays a critical role in homologous strand exchange, a key step in DNA repair through homologous recombination. It binds to single- and double-stranded DNA and exhibits DNA-dependent ATPase activity. RAD51 catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. It binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments essential for the homology search and strand exchange. Mutations in the gene can result in breast cancer, mirror movements 2, and Fanconi anemia.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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