Thermo Fisher Scientific Phospho-CHOP (Ser30) Polyclonal Antibody
Phospho-CHOP (Ser30) 폴리클로날 항체로 Western blot과 IHC(P)에서 사용 가능. Rabbit IgG 유래로 1 mg/mL 농도의 액상 형태. 세포 스트레스 조건에서 발현되는 GADD153 단백질 인식. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic phosphopeptide derived from human CHOP around the phosphorylation site of Ser30 (V-L-SP-S-D) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2555056 |
Target Information
GADD153 is a small nuclear protein capable of dimerizing with transcription factors C/EBP alpha and beta. Once dimerized, this complex inhibits the normal binding and function of C/EBP to classical binding sites. Conversely, the C/EBP–GADD153 dimer gains binding activity to other non-classical C/EBP stress-related targets. Under normal cellular conditions, this protein is not expressed at detectable levels but is highly upregulated during cellular or ER stress. Examples of GADD153-inducing stress include treatment with tunicamycin, nutrient starvation, and reducing agents that interfere with calcium flux across the ER membrane.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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