Thermo Fisher Scientific Thioredoxin 2 Polyclonal Antibody

Applications

Immunohistochemistry (Paraffin) [IHC (P)]

• Tested Dilution: 1:200–1:500

Immunocytochemistry (ICC/IF)

• Tested Dilution: 0.25–2 µg/mL

Product Specifications

Product Specific Information

Immunogen sequence:
LTSRALQTPQ CSPGGLTVTP NPARTIYTTR ISLTTFNIQD GPDFQDRVVN SETPVVVDFH AQWCGPCKIL GPRLEKMVAK QHGKVVMAKV DIDDHTDLAI EYEVSAVPTV LAMKNGDVVD KFVGIKDEDQ LEAFLKKL

Target Information

Thioredoxins (Trx) are small, multi-functional proteins with oxidoreductase activity and are ubiquitous in essentially all living cells. Trx contains a redox-active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. The two cysteine residues in the conserved active centers can be oxidized to form intramolecular disulfide bonds. Reduction of the active site disulfide in oxidized Trx is catalyzed by Trx reductase with NADPH as the electron donor. The reduced Trx serves as a hydrogen donor for ribonucleotide reductase, an essential enzyme for DNA synthesis, and acts as a general protein disulfide reductase involved in growth and redox regulation. Specific protein disulfide targets for reduction by Trx include protein disulfide-isomerase (PDI) and transcription factors such as p53, NF-κB, and AP-1. Trx also contributes to H₂O₂ removal when coupled with methionine sulfoxide reductase or isoforms of peroxiredoxins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.