Thermo Fisher Scientific Phospho-Tau (Ser202, Ser519) Polyclonal Antibody

Applications and Tested Dilution

Suggested Positive Control: JK cells

Product Specifications

Product Specific Information

A suggested positive control for Western blot is JK cells.

Target Information

Tau is a neuronal microtubule-associated protein predominantly found on axons. It promotes tubulin polymerization and stabilizes microtubules.

• The C-terminus binds axonal microtubules.
• The N-terminus binds neural plasma membrane components, acting as a linker between both.
  Tau localization defines axonal polarity within neurons. Short isoforms contribute to cytoskeletal plasticity, while longer isoforms stabilize it.

In its hyper-phosphorylated form, Tau constitutes paired helical filaments (PHF), the main component of neurofibrillary tangles in Alzheimer’s disease (AD). Hyper-phosphorylation disrupts microtubule binding, causing destabilization and neuronal degeneration.

Tau is phosphorylated by several kinases, including:

• GSK-3β
• Protein kinase A (PKA)
• Cyclin-dependent kinase 5 (cdk5)
• Casein kinase II

Hyper-phosphorylated Tau is also found in neurodegenerative diseases such as Pick’s disease, frontotemporal dementia, corticobasal degeneration, and progressive supranuclear palsy.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.