Thermo Fisher Scientific GPNMB Polyclonal Antibody
GPNMB 단백질을 인식하는 염소 유래 폴리클로날 항체. Western blot, IHC, ELISA 등 다양한 응용에 사용 가능. 인간 시료에 반응하며 항원 친화 크로마토그래피로 정제됨. 동결건조 형태로 제공되며, -20°C에서 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.5 µg/mL | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5–15 µg/mL | - |
| ELISA | 0.1–0.4 µg/mL (Detection), 0.2–0.8 µg/mL (Capture) | - |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant human Osteoactivin isoform b (Lys23–Asn486) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No Preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2607809 |
Product Specific Information
- In sandwich ELISAs, less than 2% cross-reactivity with recombinant mouse Osteoactivin is observed.
- Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
The protein encoded by this gene is a type I transmembrane glycoprotein homologous to the pMEL17 precursor, a melanocyte-specific protein. GPNMB is expressed in lowly metastatic human melanoma cell lines and xenografts but not in highly metastatic cell lines. It may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been identified for this gene.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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