Thermo Fisher Scientific Rabbit anti-Mouse IgM Secondary Antibody, AP

Applications

Tested Dilution

Western Blot (WB)

1:5,000-1:50,000

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Secondary Antibody

Conjugate

AP AP AP

View additional formats

• [Biotin ](/antibody/secondary/product-name/Mouse IgM/conjugates/Biotin/host/Rabbit/)
• [FITC](/antibody/secondary/product-name/Mouse IgM/conjugates/FITC/host/Rabbit/)
• [HRP](/antibody/secondary/product-name/Mouse IgM/conjugates/HRP/host/Rabbit/)
• Request custom conjugation

Form

Lyophilized

Concentration

0.6 mg/mL

Purification

Affinity chromatography

Storage buffer

TBS, pH 8, with 15mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient

RRID

AB_228441

Target

IgM

Antibody Form

Whole Antibody

Product Specific Information

Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.

This antibody has been successfully used in Western blot and IP applications.

Antibody Specificity: This antibody reacts with the heavy chain portion of mouse IgM. No antibody was detected against normal mouse IgG or non-immunoglobulin serum proteins. However, antibodies may cross-react with IgM from other species.

Restoration and Storage: Store product at 4°C until opened. Restore with 1.0 mL distilled water (0.6 mg/mL after restoration). Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature. To judge clarity, draw product into a pasteur pippette. Store undiluted liquid at 4°C. DO NOT FREEZE. After dilution, do not use for more than one day.

To extend the shelf-life of this product after reconstitution, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C.

Country of Origin: USA

Target Information

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.