Thermo Fisher Scientific TRAIL-R2 (DR5) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

5 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

-

Immunocytochemistry (ICC/IF)

Assay-Dependent

-

Flow Cytometry (Flow)

1 µL per million cells

-

Immunomicroscopy (IM)

Assay-Dependent

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues 388-407 (isoform 2) of human DR5 precursor. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:TRAIL-R2 (DR5),uniProtId:O14763-1,ncbiNodeId:9606,antigenRange:388-407,antigenLength:440,antigenImageFileName:PA1-41235_TRAIL-R2_DR5_O14763-1_Rabbit.svg,antigenImageFileNamePDP:PA1-41235_TRAIL-R2_DR5_O14763-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1088010

Product Specific Information

The same immunogen sequence is found in isoform 1 at amino acids 417-436.

Suggested positive control: HL-60 or Jurkat, HL-60 whole cell lysate, antigen standard for TNFRSF10B (transient overexpression lysate), Jurkat whole cell lysate.

Target Information

DR5 (Apo2, TRAIL-R2, TRICK2, KILLER) is a recently identified death domain containing receptor for TRAIL, which mediates TRAIL induced apoptosis. DR5 is a member of the TNF-receptor superfamily, and contains an intracellular death domain. DR5 can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL/APO-2L), and transduces an apoptosis signal. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by DR5. Two transcript variants encoding different isoforms of DR5 and one non-coding transcript have been found. Overexpression of DR5 induces apoptosis and activates NF-kappa-B. DR5 is expressed in a number of cell types, and to particularly high levels in lymphocytes and spleen. Diseases associated with DR5 dysfunction include squamous cell carcinoma, and diffuse infiltrative lymphocytosis syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.