Thermo Fisher Scientific GLI1 Monoclonal Antibody (1B9F8)
GLI1 단백질을 인식하는 Thermo Fisher Scientific의 Mouse Monoclonal Antibody. Western blot, IHC, ICC/IF, Flow Cytometry, ELISA 등 다양한 응용 가능. 높은 특이성과 재현성을 제공하며, 연구용으로 사용됨.
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Thermo Fisher Scientific GLI1 Monoclonal Antibody (1B9F8)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1B9F8 |
| Immunogen | Purified recombinant fragment of human GLI1 (aa 284–449) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% Sodium Azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2898442 |
Product Specific Information
This antibody has been tested in indirect ELISA.
Target Information
The GLI1 gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. Its activity and nuclear localization are negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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