Thermo Fisher Scientific Rabbit anti-Donkey IgG (H+L) Secondary Antibody, DyLight 800
Rabbit 유래의 anti-Donkey IgG (H+L) 2차 항체로 DyLight 800 형광 표지. Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용에 적합. 고순도 항원 친화성 정제 및 높은 신호 대비 제공. 연구용으로만 사용 가능.
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Applications 및 권장 희석 배수
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 |
| Immunohistochemistry (IHC) | 1:50–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:2,000 |
| Flow Cytometry (Flow) | 1:50–1:500 |
| Immunoprecipitation (IP) | Assay-dependent |
제품 사양 (Product Specifications)
| 항목 | 내용 |
|---|---|
| Species Reactivity | Donkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Donkey IgG-heavy and light chain |
| Conjugate | DyLight™ 800 |
| Excitation / Emission Max | 789 / 794 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 6.8–7.4, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2556648 |
| Target | IgG |
| Antibody Form | Whole Antibody |
추가 포맷 (Available Formats)
- AP
- Biotin
- DyLight 488
- DyLight 550
- DyLight 594
- DyLight 650
- DyLight 680
- DyLight 755
- FITC
- HRP
- Texas Red
- TRITC
- Custom conjugation available upon request
Target Information
Anti-Donkey secondary antibodies are affinity-purified antibodies with well-characterized specificity for equine (Donkey) immunoglobulins. These antibodies are useful in detection, sorting, or purification of their specified targets.
Secondary antibodies provide increased versatility, enabling use with various detection systems such as HRP, AP, or fluorescence. They can also enhance sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Typically, these antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species and can be further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to produce highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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