Thermo Fisher Scientific Phospho-EGFR (Tyr1173) Recombinant Rabbit Monoclonal Antibody (4H3F8)
EGFR의 Tyr1173 인산화 부위를 특이적으로 인식하는 재조합 토끼 단클론 항체로, Western blot 및 ELISA에 적합합니다. 인간 EGFR에 반응하며, 고순도 친화 크로마토그래피로 정제되었습니다. 연구용으로만 사용 가능합니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:1,000
ELISA
- Tested Dilution: 1 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 4H3F8 |
| Immunogen | A phospho-specific peptide corresponding to residues surrounding Y1173 of human EGFR |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.58 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS (pH 7.3), 0.05% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2912091 |
Product Specific Information
Positive test controls include BxPC-3.
The target is usually found in the following locations:
- Cell membrane
- Endoplasmic reticulum membrane
- Endosome
- Endosome membrane
- Golgi apparatus membrane
- Nucleus membrane
- Nucleus
- Secreted
- Single-pass type I membrane protein
Target Information
EGFR (epidermal growth factor receptor) is a receptor tyrosine kinase that signals in response to various growth factors. Overexpression has been linked to numerous types of cancer and EGFR is the target of both biological and small molecular therapeutics.
EGFR is encoded by the EGFR gene located on chromosome 7 in humans and belongs to the HER/ERbB family of proteins (ERbB2, ERbB3, ERbB4).
Binding of EGF or TGFα to the extracellular domain leads to EGFR dimerization and autophosphorylation of tyrosine residues in the cytoplasmic domain.
Overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, and in squamous cell carcinoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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