Thermo Fisher Scientific RNF2 Recombinant Rabbit Monoclonal Antibody (ARC0802)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

ELISA (ELISA)

1 µg/mL

-

Immunoprecipitation (IP)

0.5 µg-4 µg antibody for 200 µg-400 µg extracts of

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

ARC0802

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human RING1B/RNF2 (Q99496) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:RNF2,uniProtId:Q99496-1,ncbiNodeId:9606,antigenRange:100-200,antigenLength:336,antigenImageFileName:MA5-35379_RNF2_Q99496-1_Rabbit.svg,antigenImageFileNamePDP:MA5-35379_RNF2_Q99496-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.63 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2849280

Product Specific Information

Immunogen sequence: LRPDPNFDAL ISKIYPSRDE YEAHQERVLA RINKHNNQQA LSHSIEEGLK IQAMNRLQRG KKQQIENGSG AEDNGDSSHC SNASTHSNQE AGPSNKRTKT S

Target Information

RNF2 (E3 ubiquitin-protein ligase RING2) is a ligase that mediates monoubiquitination of Lys-119 of histone H2A (H2AK119Ub). Therefore, it plays a central role in histone code and gene regulation. The ligase activity is enhanced by BMI1/PCGF4. H2AK119Ub gives a specific tag for epigeneti transcriptional repression and participates in X chromosome inactivation of female mammals. RNF2 may be involved in the initiation of both imprinted and random X inactivation. RNF2 is also an essential component of a Polycomb group (PcG) multiprotein PRC1-like complex. This complex class is required to maintain the transcriptionally repressive state of many genes including Hox genes throughout development. The PcG PRC1 complex acts via chromatin remodeling and modification of histones and rendering chromatin heritably changed in its expressibility.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.