Thermo Fisher Scientific EMR1 Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• Reconstitute antibody with 50 µL of double distilled water (DDW).
• Store reconstituted solution at 4°C for up to 1 week.
• For long-term storage, aliquot and store at -20°C.
• Avoid multiple freeze-thaw cycles.
• Centrifuge all antibody preparations before use (10,000 × g, 5 min).
• Peptide sequence: CKQGFLSSNGQNHFK
• Homology: Mouse, Rat – 11/14 amino acid residues identical.

Target Information

F4/80 antigen is a 160 kDa cell surface glycoprotein belonging to the EGF TM7 family. It shares 68% overall amino acid identity with human EMR1 (EGF module-containing mucin-like hormone receptor 1).
Expression of F4/80 varies during macrophage maturation and activation. It is found in mature tissue macrophages such as Kupffer cells, Langerhans cells, microglia, and macrophages in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma, and spleen red pulp.
F4/80 is also expressed on some dendritic cells but is absent from macrophages in T cell areas of the spleen and lymph nodes.
Although its ligands and functions are not fully determined, studies suggest a role in the generation of efferent CD8⁺ regulatory T cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.