Thermo Fisher Scientific CD49b (Integrin alpha 2) Monoclonal Antibody (DX5), PE-Cyanine7, eBioscience
Mouse NK 및 T 세포의 CD49b 항원 검출용 PE-Cyanine7 결합 단일클론 항체. Flow cytometry에 최적화되어 있으며, 다양한 마우스 균주에서 반응. 0.5 µg/test 사용 권장, 광민감성 있으므로 암조건 보관 필요.
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Thermo Fisher Scientific CD49b (Integrin alpha 2) Monoclonal Antibody (DX5), PE-Cyanine7, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Flow Cytometry (Flow) | 0.5 µg/test | View 23 publications |
| Functional Assay | - | View 1 publication |
| Miscellaneous PubMed | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgM, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | DX5 |
| Conjugate | PE-Cyanine7 |
| Excitation / Emission Max | 569 / 780 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_469666 |
Available Formats:
- Unconjugated
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- eFluor 450
- eFluor 506
- FITC
- PE
- PE-Cyanine5
- PE-Cyanine5.5
- PE-eFluor 610
- PerCP-eFluor 710
- Custom conjugation available
Product Specific Information
Description:
The DX5 monoclonal antibody reacts with CD49b, an antigen expressed on a majority of mouse natural killer (NK) cells and a subset of T cells. DX5 reacts with all tested mouse strains including BALB/c, C57BL/6, C3H, CBA, DBA, AKR, SJL, and 129. Simultaneous staining with anti-NK1.1 mAb (PK136) shows coexpression of both markers by most NK cells, with minor DX5⁺PK136⁻ and DX5⁻PK136⁺ populations.
Applications Reported:
Reported for use in flow cytometric analysis.
Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes. Recommended ≤0.5 µg/test (100 µL final volume). Cell number may range from 10⁵–10⁸ cells/test; titration recommended for optimal performance.
Light Sensitivity:
This tandem dye is sensitive to photo-induced oxidation; protect vial and samples from light.
Fixation:
Samples can be stored in IC Fixation Buffer (Product #00-822-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency. Clone-specific performance should be empirically verified.
Excitation / Emission / Laser:
488–561 nm excitation; 775 nm emission; compatible with Blue, Green, Yellow-Green lasers.
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
CD49b (Integrin alpha 2) is a member of the integrin family. It forms a heterodimer with CD29 (Integrin beta 1) to create the VLA-2 (Integrin α2β1) complex, functioning as a receptor for laminin, collagen, and fibronectin. It plays roles in VEGF-induced angiogenesis, adhesion, and lymphocyte activation.
CD49b is expressed by NK cells, NK-T cells, monocytes, platelets, epithelial cells, and subsets of T and B cells, including CD4⁺ Tr1 regulatory cells co-expressing LAG-3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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