Thermo Fisher Scientific KLRG1 Monoclonal Antibody (2F1), Brilliant Violet 421, eBioscience

Applications

• Flow Cytometry (Flow): Tested dilution 0.25 µg/test

Product Specifications

Additional Available Formats

• Alexa Fluor 488, 532, 561, 647
• APC, APC-eFluor 780, Biotin
• Brilliant UV 395, 496, 563, 615
• Brilliant Violet 480, 605, 711
• eFluor 450, 506, FITC, Functional Grade
• Pacific Orange, PE, PE-Cyanine5/5.5/7, PE-eFluor 610
• PerCP-eFluor 710, Super Bright 436/600/645/702/780
• Request custom conjugation

Product Specific Information

The 2F1 monoclonal antibody recognizes mouse Killer cell Lectin-like Receptor G1 (KLRG1), also known as Mast cell Function-associated Antigen (MAFA). KLRG1 is a glycosylated homodimer (30–38 kDa) containing a cytoplasmic ITIM-like motif. This antigen is expressed on approximately one-third of mouse NK cells and a subset of T cells. MHC class I molecules regulate KLRG1 expression via Ly49 and SHP-1 signaling.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse splenocytes at ≤0.25 µg/test (in 100 µL volume). Cell count range: 10⁵–10⁸ cells/test. Optimize antibody titration for best performance.

Fluorochrome Information:
Brilliant Violet™ 421 (BV421) emits at 423 nm and is suitable for cytometers with a 405 nm violet laser.

When using multiple polymer dye-conjugated antibodies (e.g., Brilliant Violet™, Brilliant UV™, Super Bright™), use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific interactions.

Excitation: 407 nm
Emission: 423 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

KLRG1 (Killer cell lectin-like receptor G1, MAFA, 2F1-Ag) is a homodimeric receptor of the lectin-like type 2 transmembrane family containing ITIM motifs that interact with SH2 domains of phosphatases such as SHP-1. It functions as an inhibitory receptor expressed on NK and certain T cells, involved in immune regulation, tumor cell lysis, and antiviral responses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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