Thermo Fisher Scientific NAE1 Monoclonal Antibody (OTI1E10), TrueMAB
NAE1 단백질을 인식하는 Mouse IgG1 단일클론 항체로, Western blot과 IHC에 적합. Human에 반응하며, 무보존제 Lyophilized 형태로 제공. Alzheimer 및 세포 주기 연구용으로 활용 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:150
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1E10 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1–274 of human NAE1 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of about 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
The protein encoded by the NAE1 gene binds to the beta-amyloid precursor protein, which is involved in Alzheimer’s disease pathogenesis.
It forms a heterodimer with UBE1C, binds and activates NEDD8, and is required for cell cycle progression through the S/M checkpoint.
Three transcript variants encoding different isoforms have been identified.
For Research Use Only.
Not for use in diagnostic procedures or resale without express authorization.
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