Thermo Fisher Scientific MCM3 Recombinant Rabbit Monoclonal Antibody (JB99-35)
MCM3 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot, IHC, ICC/IF, Flow Cytometry에 사용 가능. HEK293 세포 발현, Protein A 정제, 1 mg/mL 농도. TBS buffer(40% glycerol, 0.05% BSA) 보존, -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JB99-35 |
| Immunogen | Synthetic peptide within Human MCM3 (aa 1–50) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, avoid freeze/thaw cycles, store in dark |
| Shipping Conditions | Wet ice |
| RRID | AB_2848609 |
Product Specific Information
Positive Control:
Mouse thymus tissue lysate, NIH-3T3, HeLa, 293T, HepG2, SH-SY-5Y, rat testis tissue, human tonsil tissue, human placenta tissue, mouse small intestine tissue, K562.
Target Information
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) involved in the initiation of eukaryotic genome replication. The hexameric complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and plays a role in replication fork formation and recruitment of other DNA replication-related proteins.
This protein is a subunit of the MCM2-7 complex and interacts directly with MCM5/CDC46. It is also acetylated by MCM3AP, a chromatin-associated acetyltransferase, which inhibits DNA replication initiation and cell cycle progression.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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