Thermo Fisher Scientific NLRP3 Monoclonal Antibody (768319)

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

View 7 publications 7 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

5-25 µg/mL

-

Immunohistochemistry (PFA fixed) (IHC (PFA))

-

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

-

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

8-25 µg/mL

View 2 publications 2 publications

Flow Cytometry (Flow)

2.5 µg per million cells

-

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Fungi, Human, Mouse

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

768319

Immunogen

E. coli-derived recombinant mouse NLRP3/NALP3 Met1-Arg153 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:NLRP3,uniProtId:Q8R4B8-1,ncbiNodeId:10090,antigenRange:1-153,antigenLength:1033,antigenImageFileName:MA5-23919_NLRP3_Q8R4B8-1_Rat.svg,antigenImageFileNamePDP:MA5-23919_NLRP3_Q8R4B8-1_Rat_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.5 mg/mL

Purification

Protein A/G

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2605972

Product Specific Information

In direct ELISAs, 100% cross-reactivity with recombinant human NLRP3/NALP3 is observed.

Reconstitute in sterile PBS to a final concentration of 0.5 mg/mL.

Target Information

This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site domain, and a leucine-rich repeat motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NALP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis. Mutations in this gene are associated with familial cold autoinflammatory syndrome, and neonatal-onset multisystem inflammatory disease. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Alternative 5UTR structures are suggested by available data; however, insufficient evidence is available to determine if all of the represented 5 UTR splice patterns are biologically valid.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.