Thermo Fisher Scientific NFS1 Monoclonal Antibody (OTI2H5)
NFS1 단백질을 인식하는 Thermo Fisher Scientific의 단클론 항체로, Western blot 및 IHC(P) 검증 완료. 인간 시료 반응성, Mouse IgG2b 아이소타입. 고순도 친화 크로마토그래피 정제, 안정한 PBS/BSA/glycerol 저장 버퍼 구성.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2H5 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1–299 of NFS1 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2724831 |
Target Information
NFS1 (nitrogen fixation 1), also known as NIFS or IscS (cysteine desulfurase), is a member of the class V pyridoxal-phosphate-dependent aminotransferase family.
It localizes to the cytoplasm or mitochondrion depending on cytosolic pH.
Highest expression levels are found in heart and skeletal muscle, with lower expression in liver, brain, and pancreas.
NFS1 catalyzes the removal of sulfur from cysteine to form alanine, supplying inorganic sulfur for iron-sulfur (Fe-S) clusters.
Fe-S clusters are essential cofactors in various biological processes, including electron transfer in oxidative phosphorylation, enzymatic catalysis, and DNA repair enzyme stability.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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