Thermo Fisher Scientific RPN1 Monoclonal Antibody (OTI2E3), TrueMAB
RPN1 단백질을 인식하는 Mouse IgG1 단일클론 항체로 Western blot, IHC, Flow Cytometry에 사용 가능. HEK293T 세포에서 생산된 full-length human RPN1을 면역원으로 사용. Lyophilized 형태, PBS buffer 포함, -20°C 보관.
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Thermo Fisher Scientific RPN1 Monoclonal Antibody (OTI2E3), TrueMAB
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2E3 |
| Immunogen | Full-length human recombinant RPN1 protein produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Ribophorins 1 and 2 are abundant, highly conserved glycoproteins located in the endoplasmic reticulum, involved in ribosome binding.
Mammalian oligosaccharyltransferase activity is associated with a protein complex composed of RPN1, RPN2, and an oligosaccharyltransferase protein.
RPN1 is a component of the proteasome base. The ubiquitin-like (UBL) domain of recombinant Rad23 interacts with proteasomes through the leucine-rich repeat domain of RPN1.
The RPN1 gene maps to chromosome 3, and the RPN2 gene maps to chromosome 20 by in situ hybridization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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