Thermo Fisher Scientific Granulocyte-Colony Stimulating Factor (G-CSF) Recombinant Rabbit Monoclonal Antibody (CSF3, 3166R)

Applications

• Immunohistochemistry (Paraffin) (IHC (P)): 1–2 µg/mL

Product Specifications

Product Specific Information

• For immunohistochemistry (PFA fixed): incubate antibody for 30 minutes at room temperature.
• Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.

Target Information

G-CSF (Granulocyte Colony Stimulating Factor) is a cytokine that stimulates the production and antibacterial function of neutrophils and monocytes. The human form is an 18.8 kDa protein with 175 amino acids. It is produced by activated monocytes, macrophages, endothelial cells, fibroblasts, astrocytes, osteoblasts, and bone marrow cells.
G-CSF regulates proliferation, differentiation, and activation of hematopoietic cells and is expressed by various transformed cells, including carcinoma and myeloblastic leukemia cells.
It is encoded by two distinct DNA sequences, producing a full-size high-activity and a shorter low-activity isoform. G-CSF is conserved among species and functions via its receptor on hematopoietic progenitors, granulocytes, and certain carcinoma cell lines.

Clinically, G-CSF is used for treatment of:

• Neonatal infections
• Acute myocardial infarction
• Neutropenia and sepsis
• Chronic autoimmune neutropenia
• Acute myeloid leukemia
• Sweet’s syndrome
• AIDS

It is also a marker protein for various carcinomas such as bladder cancer, and dysfunction of G-CSF is linked to Kostmann Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.