Thermo Fisher Scientific iNOS Monoclonal Antibody (CXNFT), PE-Cyanine7, eBioscience

Applications

• Flow Cytometry (Flow): Tested at 0.06 µg/test
• View 38 publications

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488, 532, 700
• APC, APC-eFluor 780
• Brilliant UV 395, 563, 615, 737
• Brilliant Violet 421, 480, 605, 786
• eFluor 450
• PE, PE-Cyanine5.5, PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

This CXNFT monoclonal antibody reacts with mouse NOS2 (inducible NOS, iNOS). Nitric oxide synthase enzymes catalyze the formation of nitric oxide from L-arginine through an NADPH- and oxygen-dependent mechanism. NOS2 is induced by bacterial endotoxins and inflammatory cytokines such as IFN-γ and TNF-α and is expressed in M1 macrophages and myeloid-derived suppressor cells.

Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Tested by intracellular staining of stimulated mouse peritoneal exudate cells using the Intracellular Fixation & Permeabilization Buffer Set (Product #88-8824-00). Recommended ≤0.06 µg/test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test. Titrate for optimal performance.

Light Sensitivity: Protect from light; tandem dye sensitive to photo-induced oxidation.
Fixation: Samples may be stored up to 3 days at 4°C in the dark in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-54).
Excitation: 488–561 nm
Emission: 775 nm
Laser Compatibility: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

iNOS (Inducible Nitric Oxide Synthase) catalyzes the oxidation of L-arginine to L-citrulline and nitric oxide (NO). NO acts as a signaling molecule involved in vasorelaxation, neurotransmission, and immune responses. iNOS expression is induced by cytokines (IFN-γ, TNF, IL-1, IL-2) and LPS, leading to sustained NO production. It is found in macrophages, hepatocytes, synoviocytes, and smooth muscle cells. Dysregulation of iNOS is associated with diseases such as achalasia and impotence.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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