Thermo Fisher Scientific Phospho-Cytokeratin 18 (Ser33) Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:1,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:50–1:100

Product Specifications

Product Specific Information

• Suggested positive control for Western blot: HT29 cells
• Suggested positive control for IHC: human breast carcinoma

Target Information

Cytokeratin 18 (Keratin 18, KRT18, type I cytoskeletal 18) belongs to the type A (acidic) subfamily of low molecular weight keratins and exists in combination with cytokeratin 8.
It is expressed in tissues from the gastrointestinal tract, respiratory tract, urogenital tract, endocrine and exocrine tissues, and mesothelial cells.
Cytokeratin 18 is primarily found in non-squamous epithelia and is present in most adenocarcinomas and ductal carcinomas, but not in squamous cell carcinomas.
Hepatocellular carcinomas express only cytokeratins 8 and 18. Mutations in the cytokeratin 18 gene are linked to cryptogenic cirrhosis.
Two transcript variants encoding the same protein have been identified for the cytokeratin 18 gene.
Cytokeratin subtype expression patterns assist in distinguishing epithelial malignancies and are valuable tools in immunohistochemistry, cytopathology, and flow cytometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.