Thermo Fisher Scientific POLR2A Monoclonal Antibody (OTI3C8), TrueMAB

Applications and Tested Dilution

Product Specifications

Target Information

DNA-directed RNA polymerase II subunit RPB1 (POLR2A) is a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. POLR2A is the largest subunit and a catalytic component of RNA polymerase II, which synthesizes mRNA precursors and many functional non-coding RNAs. It forms the polymerase active center together with the second largest subunit.

RNA polymerase II (Pol II) is the central component of the basal transcription machinery, composed of mobile elements that move relative to each other. RPB1 contributes to the core element with the large cleft, clamp element, and jaws that engage the DNA template. During transcription initiation, the promoter’s single-stranded DNA template is positioned within the central active site cleft of Pol II. The bridging helix from RPB1 promotes translocation of Pol II by acting as a ratchet mechanism.

Transcription elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of RPB1, which serves as a platform for assembly of factors regulating transcription initiation, elongation, termination, and mRNA processing. Gene expression regulation depends on the balance between methylation and acetylation levels of CTD lysines.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.