Phosphate Sensor

The Phosphate Sensor is a highly sensitive reagent for the detection of inorganic phosphate, either in real-time kinetic or end-point자세히 알아보기

The Phosphate Sensor is a highly sensitive reagent for the detection of inorganic phosphate, either in real-time kinetic or end-point mode. This product is a purified form of recombinant E. coli phosphate-binding protein labeled with the fluorophore MDCC, which is sensitive to changes in its environment. Binding of inorganic phosphate by Phosphate Sensor is rapid and tight (Kd ∼ 0.1 μM), resulting in a large increase in fluorescence. The Phosphate Sensor detects phosphate in the high-nanomolar to low-micromolar range, which is 100-fold more sensitive than malachite green. This reagent provides you with a sensitive assay for detecting enzymes that produce inorganic phosphate either directly or indirectly through coupled reactions, such as phosphatases, ATPases, GTPases, and phosphodiesterases.

사양

용도(장비)Microplate Reader

타겟GTPases, ATPases, Phosphatases, Phosphodiesterases

용도(애플리케이션)Fluorescence Intensity

어세이Phosphatase Assay

검출 방법Fluorescence

라벨 또는 염료MDCC

제품 유형Phosphate Sensor

수량10 nmol

형식384-well plate

여기/방출430⁄450

판독Kinetic

배송 조건Dry Ice

Unit SizeEach