Thermo Fisher Scientific PNPase Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.2-1 µg

-

Immunohistochemistry (IHC)

5 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide directed towards the middle region of human PNPT1

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS with 2% sucrose

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2606448

Product Specific Information

Peptide sequence: CGGSLALMDS GVPISSAVAG VAIGLVTKTD PEKGEIEDYR LLTDILGIED

Sequence homology: Cow: 79%; Dog: 79%; Guinea Pig: 86%; Horse: 79%; Human: 100%; Mouse: 93%; Rat: 86%

Target Information

PNPT1 (polyribonucleotide nucleotidyltransferase 1, mitochondirla) is a RNA-binding protein implicated in numerous RNA metabolic processes. It catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3-to-5 direction. PNPT1 is a component of the mitochondrial degradosome (mtEXO) complex that degrades 3overhang double-stranded RNA with a 3-to-5` directionality in an ATP-dependent manner. It is required for correct processing and polyadenylation of mitochondrial mRNAs. It plays a role as a cytoplasmic RNA import factor, in mitochondrial morphogenesis and respiration, regulation of expression of the electron transport chain, regulation of the stability of specific mature miRNAs in melanoma cells, and RNA cell surveilance. Mutations of the gene can result in combined oxidative phosphorylation deficiency 13 (COXPD13) and deafness, autosomal recessive, 70 (DFNB70).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.