Thermo Fisher Scientific S100A4/Metastasin/Calvasculin Recombinant Mouse Monoclonal Antibody (rS100A4, 1481)
S100A4 단백질을 인식하는 Thermo Fisher Scientific의 재조합 마우스 단클론 항체로, Western blot, IHC, ICC/IF, Flow Cytometry 등에 사용 가능. 인간 및 마우스 반응성, HEK293 발현 시스템, Protein A/G 정제, 4°C 보관.
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Thermo Fisher Scientific S100A4/Metastasin/Calvasculin Recombinant Mouse Monoclonal Antibody (rS100A4, 1481)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | rS100A4, 1481 |
| Immunogen | Recombinant fragment (around aa 2–101) of human S100A4 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
The protein encoded by this gene is a member of the S100 family of proteins containing two EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of various cell types and are involved in regulating cellular processes such as cell cycle progression and differentiation.
S100 genes include at least 13 members clustered on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants encoding the same protein have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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