Thermo Fisher Scientific LRRC25 Monoclonal Antibody (OTI1A2), TrueMAB
Human LRRC25 단백질을 인식하는 마우스 단일클론 항체로 Western blot, IHC, Flow cytometry에 사용 가능. TrueMAB 포맷으로 높은 특이성과 재현성을 제공. 동결건조 형태로 보관 용이하며 연구용으로 최적화됨.
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Thermo Fisher Scientific LRRC25 Monoclonal Antibody (OTI1A2), TrueMAB
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1A2 |
| Immunogen | Full length human recombinant LRRC25 protein produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
LRRC25 plays a role in inhibiting RLR-mediated type I interferon signaling by targeting DDX58/RIG-I for autophagic degradation.
It interacts with ISG15-associated DDX58 to promote interaction with the autophagic cargo receptor p62/SQSTM1, mediating DDX58 degradation via selective autophagy (PubMed:29288164).
Additionally, LRRC25 inhibits NF-kappa-B signaling and inflammatory response by promoting degradation of p65/RELA. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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