Thermo Fisher Scientific RAP1GAP Polyclonal Antibody
RAP1GAP 단백질을 인식하는 Thermo Fisher Scientific의 폴리클로날 항체로, WB 및 IHC(P) 실험에 적합합니다. Human 및 Mouse 반응성을 가지며, 항원 친화 크로마토그래피로 정제되었습니다. 고순도(>95%)이며 연구용으로만 사용됩니다.
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Thermo Fisher Scientific RAP1GAP Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 525–570 of Human RAP1GAP |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C (short term); for long-term storage, store at -20°C and avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2803487 |
Product Specific Information
- Purity is >95% by SDS-PAGE.
Target Information
Rap1GAP specifically stimulates GTP hydrolytic activity of the monomeric G protein Rap1. Physical interaction between G alpha z (Gi family) and Rap1GAP blocks regulators of G protein signaling from stimulating GTP hydrolysis of the alpha subunit, and attenuates activated G alpha z inhibition of adenylyl cyclase.
Rap1GAP is expressed in the brain, kidney, and pancreas, and may act as a signal integrator coordinating Gz and Rap1 signaling.
A novel isoform, Rap1GAPII, binds specifically to G alpha z. Stimulation of the Gi-coupled M2 Muscarinic receptor translocates Rap1GAPII from the cytosol to the membrane, decreases GTP-bound Rap1, and activates ERK/MAPK signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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