Thermo Fisher Scientific 4E-BP2 Polyclonal Antibody
Rabbit polyclonal antibody against human 4E-BP2 (EIF4EBP2) for WB and ELISA. Recognizes amino acids 61–120 of EIF4EBP2. Supplied as liquid, 1.45 mg/mL, affinity purified, in PBS with 50% glycerol. For research use only.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| ELISA | 1 µg/mL |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing amino acids 61–120 of human EIF4EBP2 (NP_004087.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.45 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | −20 °C, avoid freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2805519 |
Product Specific Information
- Immunogen sequence: DRRNSPMAQT PPCHLPNIPG VTSPGTLIED SKVEVNNLNN LNNHDRKHAV GDDAQFEMDI
- Positive samples: HeLa, Jurkat
Target Information
PHAS-II (eIF4E-binding protein II) is involved in translation initiation and is highly expressed in liver and kidney. PHAS proteins bind to eIF-4E, the mRNA cap-binding protein, inhibiting translation of capped mRNA. Phosphorylation at specific sites promotes dissociation of PHAS/eIF-4E complexes, enabling eIF-4E to bind eIF-4G (p220) and enhance translation initiation. The mTOR/p70S6K pathway regulates PHAS-I and -II, linking these proteins to control of protein synthesis and cell proliferation.
Safety Information
⚠ WARNING: This product can expose you to chemicals including mercury, known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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