Thermo Fisher Scientific CD46 Monoclonal Antibody (8E2), PE, eBioscience

Applications

Tested Dilution

Publications

Immunohistochemistry (Frozen) (IHC (F))

-

View 1 publication 1 publication

Flow Cytometry (Flow)

5 µL (0.5 µg)/test

View 4 publications 4 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

8E2

Conjugate

PE PE PE

View additional formats

• PerCP-eFluor 710
• Request custom conjugation

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1963584

Product Specific Information

Description: The 8E2 monoclonal antibody reacts with human CD46 (membrane cofactor protein). CD46 is a transmembrane glycoprotein consisting of four regularly expressed isoforms, giving an electrophoretic profile of two heterogeneous protein species of 51-58 kDa (lower band) and 59-68 kDa (upper band). CD46 is expressed on the majority of nucleated cells, and functions as a regulator of complement function by binding complement components C3b and C4b, which serves to protect the host from autologous complement attack. CD46 has been called a pathogen magnet as it is known to play a role in the entry of several pathogens including strains of measles virus, human herpes virus type 6, group A streptococci and Neisseria.

Cross-linking TCR on naive human peripheral blood CD4+ T cells together with CD46, rather than CD28, induces more robust proliferation than achieved with stimulation by the costimulatory molecule CD28, as well as other qualitative differences. There is evidence to suggest that stimulation of TCR and CD46 activates natural CD4+CD25+ T regs, which are non-proliferating, but produce high amounts of granzyme A, and kill activated CD4+, CD8+, CD14+ monocytes, immature dendritic cells and mature dendritic cells.

Applications Reported: This 8E2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 8E2 antibody has been pre-titrated and tested by flow cytometric analysis of nomal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD46 (MCP, membrane cofactor protein) is a multifunctional cell surface transmembrane protein that binds and inactivates C3b and C4b complement fragments, regulates T cell-induced inflammatory responses by either inhibiting (CD46-1 isoform) or increasing (CD46-2 isoform) the contact hypersensitivity reaction. CD46 also serves as a receptor for several human pathogens (both bacteria and viruses), and its ligation alteres T lymphocyte polarization toward antigen-presenting cells or target cells, inhibiting lymphocyte function. CD46 is a protector of placental tissue and is also expressed on the inner acrosomal membrane of spermatozoa.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.