Thermo Fisher Scientific Integrin beta 5 Monoclonal Antibody (KN52), PE, eBioscience
인테그린 베타 5를 인식하는 KN52 단클론 항체로, PE 형광 표지된 제품입니다. 인간, 햄스터, 마우스에서 반응하며 Flow Cytometry에 최적화되어 있습니다. 세포 이동 억제 기능을 가지며, 4°C 암조건 보관 권장.
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Thermo Fisher Scientific Integrin beta 5 Monoclonal Antibody (KN52), PE, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Flow Cytometry (Flow) | 5 µL (0.06 µg)/test | View 3 publications |
| Neutralization (Neu) | - | View 1 publication |
| Functional Assay (Functional) | - | View 1 publication |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Hamster, Human, Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | KN52 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10854720 |
Additional Formats
Product Specific Information
The KN52 antibody reacts with human integrin beta 5, an approximately 100,000 Mr (reduced) and 95,000 Mr (nonreduced) member of the integrin family. Only a single alpha chain, the alpha v subunit, associates with integrin beta 5 to form the vitronectin receptor complex.
Integrin alpha v/beta 5 complex also binds to the basic domain of Tat (sequence RKKRRQRRR). Integrin beta 5 is found on carcinoma, hepatoma, and fibroblast cell lines, but absent from lymphoblastoid cells and platelets.
The KN52 monoclonal antibody has been shown to inhibit migration.
Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-titrated and tested by flow cytometric analysis of CHO, A549, and normal human peripheral blood cells. Recommended use: 5 µL (0.06 µg) per test (final volume 100 µL). Cell number range: 10⁵–10⁸ cells/test.
Excitation: 488–561 nm
Emission: 578 nm
Laser Compatibility: Blue, Green, Yellow-Green Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
Integrins are extracellular matrix (ECM) receptor proteins located on cell surfaces. They are heterodimers composed of alpha and beta transmembrane glycoprotein subunits. About 22 different integrins exist, each with unique alpha/beta combinations.
Integrins are abundant at the cell surface and bind ligands with low affinity, requiring clustering for effective ECM binding. They interact with various ligands including laminin. Each integrin features a large N-terminal extracellular domain binding ECM ligands and a small C-terminal cytoplasmic domain mediating actin cytoskeleton interaction and signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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