Invitrogen™

pEF6/V5-His TOPO™ TA Expression Kit

The pEF6/V5-His TOPO™ TA Expression Kit is designed for high-level mammalian expression from the powerful human EF-1 α promoter with자세히 알아보기

The pEF6/V5-His TOPO™ TA Expression Kit is designed for high-level mammalian expression from the powerful human EF-1 α promoter with the added convenience of one-step TOPO™ Cloning. The pEF6/V5-His-TOPO™ vector includes:

Enhancer/promoter elements from the human elongation factor 1α subunit (hEF-1α) for high-level expression in mammalian cells

• The Blasticidin resistance gene for rapid selection of stable mammalian cell lines
• A C-terminal V5 epitope for efficient detection with an Anti-V5 Antibody
• A C-terminal polyhistidine (6xHis) sequence for rapid purification with nickel-chelating resin and detection with an Anti-His(C-term)
• Antibody

사양

구성 또는 유도성 시스템Constitutive

배달 유형Transfection

프로모터EF-1α

제품 유형TOPO TA Expression Kit

선택 제제(진핵)Blasticidin

분열EK (Enterokinase) Recognition Site

단백질 태그His Tag (6x), V5 Epitope Tag

클로닝 방법TOPO-TA

수량20 reactions

벡터pEF, TOPO-TA Vectors

제품라인TOPO™

용도(애플리케이션)Constitutive Expression

Unit SizeEach