Invitrogen™

Expressway™Lumio™ Expression and Detection System, without vector

The Expressway™ Lumio™ Expression and Detection System takes advantage of the Lumio™ recognition sequence, a small, six amino acid sequence자세히 알아보기

The Expressway™ Lumio™ Expression and Detection System takes advantage of the Lumio™ recognition sequence, a small, six amino acid sequence (Cys-Cys-Pro-Gly-Cys-Cys). The Lumio™ detection reagent binds the recognition sequence with high specificity and affinity, resulting in a bright fluorescent signal for real-time protein production analysis and immediate in-gel protein detection. In addition, Expressway`s specialized E. coli lysate, derived from a _sly_D mutant, eliminates nonspecific binding of the Lumio™ Green Detection Reagent to the endogenous SlyD protein and provides optimal background for detection of recombinant proteins (Figure 1). The Lumio™ Green Detection Kit is included in the system for real-time detection of protein synthesis as well as easy in-gel protein detection. The Lumio™ vectors (Figures 2 and 3) also feature:

• _att_R sites for efficient recombination with any _att_L-flanked Gateway™ entry vector
• N-terminal Lumio™ tag (pEXP3-DEST vector) with TEV cleavage site for efficient removal of the Lumio™ sequence following purification
• C-terminal Lumio™ tag (pEXP4-DEST vector) for easy, and immediate in-gel detection
• T7 promoter, ribosome binding site, and T7 terminator optimally spaced for cell-free protein expression

사양

제품 유형Lumio Expression and Detection System

검출 위치In-Gel Detection

발현 단백질 유형Water Soluble Protein

발현 시스템Cell-Free

발현 메커니즘Cell-Free Expression

수량1 kit

제품라인Expressway™, Lumio™

Unit SizeEach