Thermo Fisher Scientific alpha Tubulin Monoclonal Antibody (YL1/2)
alpha Tubulin을 인식하는 YL1/2 클론의 Rat 모노클로날 항체로, 다양한 종에서 반응하며 WB, ICC/IF, ELISA 등에 적합합니다. Tyr-Tubulin에 특이적으로 결합하며 로딩 컨트롤로 사용 가능합니다. 단백질 G로 정제된 액상 형태로 4°C 또는 -20°C에서 보관합니다.
- 카탈로그번호
- MA180017
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific alpha Tubulin Monoclonal Antibody (YL1/2)
Thermo Fisher Scientific alpha Tubulin Monoclonal Antibody (YL1/2)
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 2 µg/mL | View 23 publications |
| Immunohistochemistry (IHC) | - | View 14 publications |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | - | View 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent | View publications |
| Immunocytochemistry (ICC/IF) | 2 µg/mL | View 38 publications |
| Flow Cytometry (Flow) | - | View 1 publication |
| ELISA | 1:100–1:1,000 | View publications |
| Immunoprecipitation (IP) | Assay-dependent | View publications |
| Radioimmune Assays (RIA) | Assay-dependent | View publications |
| Blocking Assay (BLOCK) | - | View 1 publication |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Fruit fly, Human, Mouse, Plant, Pig, Rabbit, Rat, Xenopus, Yeast |
| Published Species | Arthropod, Bacteria, Cnidarian, Dog, Fungi, Human, Insect, Mouse, Non-human primate, Plant, Protozoa, Rabbit, Rat |
| Host / Isotype | Rat / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | YL1/2 |
| Immunogen | Yeast tubulin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2210201 |
Product Specific Information
This clone recognizes the alpha subunit of tubulin, specifically binding tyrosylated Tubulin (Tyr-Tubulin).
The epitope recognized by this antibody is a linear sequence requiring an aromatic residue at the C terminus, with two adjacent negatively charged amino acids (Glu-Glu-Tyr in Tyr-Tubulin).
MA1-80017 is predicted to react with amphibia, birds, and echinoderm based on sequence homology.
Does not react with Nephrotoma suturalis.
This antibody is suitable for use as a loading control.
It has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope.
These sequence requirements may lead to cross-reactivity with other proteins in certain cases, including E. coli rec A and oxidized actin.
Target Information
Alpha Tubulin is part of a heterodimer consisting of alpha and beta tubulin subunits, each approximately 55 kDa.
The alpha-beta tubulin heterodimer forms the basic building block of microtubules, which are cylindrical filamentous structures present in almost all eukaryotic cells.
Microtubules function as structural supports, intracellular transport lines, and play key roles in mitosis.
They are composed mainly of alpha and beta tubulins, while gamma tubulin is critical for microtubule nucleation.
Multiple alpha and beta tubulin genes are highly conserved among species.
The alpha tubulin gene shows high similarity to the mouse and rat Tuba1 gene.
Northern blotting studies indicate predominant expression in morphologically differentiated neurologic cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.