Thermo Fisher Scientific SH3BP1 Monoclonal Antibody (OTI2F4), TrueMAB
SH3BP1 단백질을 인식하는 Mouse 모노클로날 항체로, Western blot 및 IHC(P)에서 검증됨. Human 및 Mouse 시료에 반응. 동결건조 형태로 제공되며, PBS 버퍼(8% trehalose) 내 보존. 연구용으로 Rac 관련 단백질의 GAP 활성 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2F4 |
| Immunogen | Full length human recombinant protein of human SH3BP1 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
SH3BP1 binds differentially to SH3 domains of proteins involved in signal transduction pathways.
It preferentially binds to ABL1, SRC, and GRB2, showing GAP activity for Rac-related proteins but not for Rho- or Ras-related proteins.
Inhibits PDGF-induced membrane ruffling mediated by Rac.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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