Thermo Fisher Scientific HAP1 Monoclonal Antibody (1B6)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-1.0 µg/mL

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

0.5-1.0 µg/mL

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

0.5-1.0 µg/mL

-

Immunocytochemistry (ICC/IF)

Assay-Dependent

-

Flow Cytometry (Flow)

1 µg per million cells

-

Immunoprecipitation (IP)

1:10-1:500

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1B6

Immunogen

Fusion protein from rat HAP1.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS with 50% glycerol

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2232841

Product Specific Information

In Western blot bands can be seen ~95 kDa for isoform A and ~110 kDa for isoform B.

Suggested positive control: rat brain.

Target Information

Huntington`s disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons, is caused by an expansion of a polyglutamine tract in the HD protein huntingtin. HAP1 was initially identified through a two-hybrid library screening; the binding of HAP1 to huntingtin correlated with the expansion of the polyglutamine tract. HAP1 also interacts with two cytoskeletal proteins (dynactin and pericentriolar autoantigen protein 1), suggesting that HAP1 may play a role in vesicular trafficking or organelle transport. HAP1 is also involved with the huntingtin-enhanced BDNF transport along the cellular microtubles. Attenuation of this process led to the loss of neurotrophic support and neuronal toxicity, which suggests that loss of this function might contribute to pathogenesis. Several alternatively spliced isoforms have been described for HAP1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.