Thermo Fisher Scientific CD273 (B7-DC) Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

• In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse PD-L2 is observed.
• Less than 1% cross-reactivity with recombinant human B7-1, B7-2, B7-H1, B7-H2, B7-H3, B7-H3b, and recombinant rat B7-1.
• Reconstitute at 0.2 mg/mL in sterile PBS.
• Endotoxin level: <0.10 EU per 1 µg of antibody (LAL method).

Target Information

Programmed death-ligand 2 (PD-L2), also known as B7-DC, is a member of the B7 ligand family within the immunoglobulin superfamily. Along with PD-L1, it acts as a ligand for PD-1. PD-L2 is primarily expressed in dendritic cells but can be induced in various immune and non-immune cells depending on the microenvironment. Expression is upregulated by IL-4 and, to a lesser degree, by TNF-alpha and IFN-gamma.

PD-L2 has a 2–6 times higher affinity for PD-1 compared to PD-L1. PD-1 and its ligands function as inhibitory immune checkpoint molecules, providing negative feedback during immune responses. Ligation of PD-L2 or PD-L1 inhibits T cell activation, proliferation, and cytokine secretion (e.g., IFN-gamma, IL-10). However, PD-L2 can also stimulate T cell responses independently of PD-1, suggesting additional receptors.

Recent studies show PD-L2 binds to repulsive guidance molecule b (RGMb), influencing respiratory tolerance through BMP and neogenin signaling pathways. The human PD-L2 monomer consists of a 201-amino-acid extracellular domain, a 21-amino-acid transmembrane domain, and a 32-amino-acid cytoplasmic domain.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.