Thermo Fisher Scientific EZH2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

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ELISA (ELISA)

1 µg/mL

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Immunoprecipitation (IP)

0.5 µg-4 µg antibody for 200 µg-400 µg extracts of

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Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 158-261 of EZH2/KMT6 (NP_001190176.1).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2.21 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2914066

Product Specific Information

Positive test controls include: Jurkat, HT-29, HepG2, NIH/3T3. The target is usually found in the following locations: Nucleus.

Immunogen sequence: HGDRECGFIN DEIFVELVNA LGQYNDDDDD DDGDDPEERE EKQKDLEDHR DDKESRPPRK FPSDKIFEAI SSMFPDKGTA EELKEKYKEL TEQQLPGALP PECT

Target Information

EZH2 (Histone-lysine N-methyltransferase) is a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. EZH2 may play a role in the hematopoietic and central nervous systems. Mutations affecting EZH2 can cause Weaver syndrome (WVS).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.