Thermo Fisher Scientific CD61 Monoclonal Antibody (HMbeta3-1), APC

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-dependent

View 5 publications 5 publications

Miscellaneous PubMed (Misc)

-

View 2 publications 2 publications

Product Specifications

Published species

Mouse

Host/Isotype

Armenian hamster / IgG

Class

Monoclonal

Type

Antibody

Clone

HMbeta3-1

Immunogen

Mouse/Rat CD61.

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_10375168

Product Specific Information

According to the literature this antibody recognizes the CD61 antigen. Use dim light during handling, incubation with cells and prior to analysis. It is recommended that cells be analyzed within 18 hours of staining. If the reagent is being diluted, it is recommended that only the quantity to be used within one week be diluted.

The amount of antibody is determined by measuring the optical density using a spectrophotometer. The antibody titer is verified by immunofluorescent staining and flow cytometric analysis.

Every lot is tested by flow cytometry using platelets isolated from murine peripheral blood essentially according to the procedure contained in Current Protocols in Cytometry (2002) 6.10.1 - 6.10.17. From this testing it is recommended that between 0.1 and 0.25 µg of antibody be used per 1 x 10^6 cells in a 100 µL staining volume.

Target Information

CD61 (GPIIIa, ITGB3) is a glycoprotein found on megakaryocytes, platelets and their precursors. CD61 antigen plays a role in platelet aggregation and also as a receptor for fibrinogen, fibronectin, von Willebrand factor and vitronectrin. CD61 is a 105 kDa glycoprotein that associates with either the alpha 11b integrin (CD41) or the alpha V integrin (CD51) at the cell surface. CD61 is expressed on platelets and megakaryocytes in association with CD41, and on endothelial cells, monocytes, platelets and osteoclasts in association with CD51. CD61 is a receptor for fibrinogen, fibronectin, Von Willebrand Factor, vitronectin and thrombospondin. The CD61 protein product is composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. CD61 is found along with the alpha IIb chain in platelets. Like other integrins, CD61 is known to participate in cell adhesion as well as cell-surface mediated signaling. Diseases associated with CD61 dysfunction include Glanzmann Thrombasthenia and Platelet type-16 Bleeding Disorder.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.