Gibco™

SILAC RPMI 1640 Flex Media, no glucose, no phenol red

SILAC RPMI 1640 Medium is RPMI 1640 basal cell culture medium without L-arginine, L-glutamine, and L-lysine for use during SILAC자세히 알아보기

SILAC RPMI 1640 Medium is RPMI 1640 basal cell culture medium without L-arginine, L-glutamine, and L-lysine for use during SILAC protein labeling with stable isotope-labeled lysine and/or arginine. RPMI 1640 Medium is widely used for supporting the growth of many different mammalian cells. Cells successfully cultured in RPMI-1640 include HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of Gibco™ RPMI 1640 modifications for a range of applications. Find the right modification using the media selector tool.

This RPMI 1640 Medium is modified as follows:

With	Without
	• Glucose
	• Phenol red
	• HEPES
	• L-arginine
	• L-glutamine
	• L-lysine

RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagles Minimal Essential Medium or Dulbeccos Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). SILAC protein labeling experiments should be conducted using Gibco™ Dialyzed FBS. RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5-10% CO2 environment to maintain physiological pH.

cGMP Manufacturing and Quality System
SILAC RPMI 1640 Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

사양

세포 유형Mammalian Cells

Culture TypeMammalian Cell Culture

형태Liquid

제품 유형RPMI 1640

Serum LevelStandard Serum Supplementation

유통 기한12 Months

첨가제 없음No Glucose, No Glutamine, No HEPES, No Phenol Red, No Sodium Pyruvate, No Arginine, No Lysine

제품라인Gibco™

수량500 mL

Unit SizeEach