Thermo Fisher Scientific EDD1 Polyclonal Antibody
인간 EDD1 단백질을 인식하는 Rabbit Polyclonal 항체로 Western blot, IHC, IP 등 다양한 응용에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며 1 mg/mL 농도의 액상 형태로 제공. FFPE 조직에서는 citrate buffer pH 6.0 전처리 권장. 연구용 전용 제품.
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Applications and Tested Dilutions
| Application | Tested Dilution / Amount |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 600 and 650 of human E3 Ubiquitin Protein Ligase, HECT Domain Containing, 1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Phosphate/tris citrate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
- Based on 100% sequence identity, this antibody is predicted to react with Rat
Target Information
EDD (E3 identified by Differential Display) is a progestin-regulated gene isolated from T-47D human breast cancer cells. Based on sequence homology, EDD appears to be a human homolog of the Drosophila hyperplastic discs (hyd) gene, a tumor suppressor required for imaginal disc growth control.
EDD contains a HECT domain in the carboxy terminus. HECT domain-containing proteins function as ubiquitin-protein ligases (E3 enzymes). EDD binds to ubiquitin and, like other HECT family proteins, may function as an E3 ubiquitin-protein ligase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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