Takara Guide-it recombinant Cas9

Guide-it recombinant Cas9 is a recombinant wild-type Streptococcus pyogenes Cas9 nuclease modified with a C-terminal nuclear localization signal (NLS) for use in CRISPR/Cas9-mediated gene-editing experiments. Electroporation of mammalian cells with Cas9-sgRNA ribonucleoprotein complexes allows you to minimize off-target effects while facilitating a fast turnaround for highly efficient gene-editing experiments.

Overview

• The Cas9 protein solution is sterile and well-tolerated by mammalian cells
• Highly efficient editing when combined with our Guide-it Complete sgRNA Screening System
• Minimizes off-target effects—better than plasmid-based delivery systems
• Works well in hard-to-transfect cell lines

More Information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.