Thermo Fisher Scientific p27Kip1 (Mitotic Inhibitor/Suppressor Protein) Recombinant Rabbit Monoclonal Antibody (KIP1/1355R)
Recombinant rabbit monoclonal antibody targeting p27Kip1, a key cell cycle regulator. Validated for IHC applications with high specificity. Produced in HEK293 cells and purified via Protein A/G. Supplied as a liquid form for reliable research use.
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Applications and Tested Dilutions
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 2 µg/mL
Immunohistochemistry (PFA fixed) (IHC (PFA))
- Tested Dilution: 1–2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | KIP1/1355R |
| Immunogen | Recombinant full-length human CDKN1B protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
p27 Kip1 functions as a negative regulator of G1 phase progression and may mediate TGFβ-induced G1 arrest. It is an important regulator of cell cycle progression and a potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. p27 Kip1 forms complexes with cyclin D-CDK4, contributing to assembly, stability, and modulation of CCND1-CDK4 activation. Depending on its phosphorylation state, p27 Kip1 can act as either an inhibitor or activator of cyclin D-CDK4 complexes. This protein (~27 kDa) undergoes CDK-dependent phosphorylation and SCF-mediated ubiquitination, which are essential for the transition from quiescence to proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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