Thermo Fisher Scientific N-Cadherin Polyclonal Antibody
N-Cadherin 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody입니다. Western blot 및 IHC(Frozen)에서 검증됨. 항원 친화 크로마토그래피로 정제된 고순도 항체로, 세포 간 칼슘 의존적 접착 연구에 적합합니다.
- 카탈로그번호
- ANR-082-xxxxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific N-Cadherin Polyclonal Antibody
Thermo Fisher Scientific N-Cadherin Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | (C)DTVEPDAIKPVGIR, corresponding to amino acid residues 794–807 of mouse CDH2 (Intracellular) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.85 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Reconstitution: Add 25 µL, 50 µL, or 0.2 mL double distilled water (DDW), depending on the sample size.
The antibody ships as a lyophilized powder at room temperature. Upon arrival, store at -20°C.
Reconstituted solution can be stored at 4°C for up to 1 week. For longer storage, aliquot and store at -20°C.
Avoid multiple freeze-thaw cycles. Centrifuge all antibody preparations before use (10,000 × g, 5 min).
Target Information
N-cadherin is a 140 kDa transmembrane protein that mediates calcium-dependent intercellular adhesion.
It plays a critical role in morphogenetic movements during development and regulates cell surface adhesion through homotypic interactions.
Its function depends on association with the actin-cytoskeleton via catenin proteins, with stability modulated by phosphorylation of beta-catenin.
N-cadherin consists of five extracellular cadherin repeats, a transmembrane region, and a conserved cytoplasmic tail.
It is essential for gastrulation and establishment of left-right asymmetry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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